How useful are FMOs for setting gates? Many times the FMO negative population boundary is much lower than in the fully stained sample. I would refer to Perfetto et al, 2012 for more details – I try to go as precise as possible and would rather say +/- 5% or less. When you said that you adjust your voltage to obtain the same MFIs for your beads, what is your “acceptable” range? Is +\- 10% is ok? This is a difficult question to answer as the acceptable range of variation depends on the particular instrument.
Note: these answers are based on the verbal Q&A of the webinar and are not meant to be fully exhaustive references. Questions compiled and answered by Sabine Ivison (from CAN-ASC) and Florian Mair. Learn More Q&A Summary “A step-by-step guide to generating high parameter flow cytometry data” by Florian Mair, PhD For more information on our CAN-ASC Webinar Series, visit our Webinars page.
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